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CD326 Antibody
SKU: orb44756
Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 4
| Tested Applications | FC, ICC, IHC-Fr, IHC-P, IP, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | VU-1D9 |
| Immunogen | Small cell lung carcinoma cell line H69. |
| Target | CD326 |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−EPCAM, GA733-2, EGP314, KSA, KS 1/4 antigen, Trop-1, M4S1, DIAR5, MIC18, TROP1
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Separation of MCF-7 cells (red-filled) from SP2 cells (black-dashed) in flow cytometry analysis (surface staining) of cell lines stained using anti-human CD326 (VU-1D9) purified antibody (concentration in sample 6 µg/ml) GAM APC.
Flow cytometry surface staining pattern of MCF-7 cells stained using anti-human CD326 (VU-1D9) purified antibody (concentration in sample 6 µg/ml) GAM APC.
Immunohistochemistry staining of human prostate (paraffin sections) using anti-CD326 (VU-1D9).
Western blotting analysis of human CD326 using mouse monoclonal antibody VU-1D9 on lysates of MCF-7 cells and HEK293T cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD326 was detected around 40 kDa.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
ICC
Immunocytochemistry
IP
Immunoprecipitation
CD326 Antibody (orb44756)
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