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Anti-Hu CD326 Purified
Catalog Number: orb44756
| Catalog Number | orb44756 |
|---|---|
| Category | Antibodies |
| Description | Mouse Monoclonal antibody againts EpCAM |
| Clonality | Monoclonal |
| Clone Number | VU-1D9 |
| Tested applications | FC, ICC, IHC-Fr, IHC-P, IP, WB |
| Reactivity | Human |
| Isotype | Mouse IgG1 |
| Immunogen | Small cell lung carcinoma cell line H69. |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | CD326 |
| Entrez | 4072 |
| UniProt ID | P16422 |
| RRID | AB_10996006 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | anti Adenocarcinoma associated antigen antibody, a Read more... |
| Note | For research use only |
| Application notes | Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/ml; positive control: colon epithelium, antigen retrieval: heat (sodium citrate) + trypsin. Immunocytochemistry: Recommended dilution: 4 μg/ml. Western blotting: Recommended dilution: 1-4 μg/ml. Flow cytometry: Recommended dilution: 1-3 μg/ml. Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein in 1 ml lysate. |
| Expiration Date | 12 months from date of receipt. |
Separation of MCF-7 cells (red-filled) from SP2 cells (black-dashed) in flow cytometry analysis (surface staining) of cell lines stained using anti-human CD326 (VU-1D9) purified antibody (concentration in sample 6 µg/ml) GAM APC.
Flow cytometry surface staining pattern of MCF-7 cells stained using anti-human CD326 (VU-1D9) purified antibody (concentration in sample 6 µg/ml) GAM APC.
Immunohistochemistry staining of human prostate (paraffin sections) using anti-CD326 (VU-1D9).
Western blotting analysis of human CD326 using mouse monoclonal antibody VU-1D9 on lysates of MCF-7 cells and HEK293T cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD326 was detected around 40 kDa.
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