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Anti-Hu CD326 Purified
Catalog Number: orb43595
| Catalog Number | orb43595 |
|---|---|
| Category | Antibodies |
| Description | Mouse Monoclonal antibody againts EpCAM |
| Clonality | Monoclonal |
| Clone Number | 323/A3 |
| Tested applications | FC, ICC, IHC-P, IP, WB |
| Reactivity | Human |
| Isotype | Mouse IgG1 |
| Immunogen | Human breast cancer MCF-7 cells |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | CD326 |
| Entrez | 4072 |
| UniProt ID | P16422 |
| RRID | AB_10993168 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | anti Adenocarcinoma associated antigen antibody, a Read more... |
| Note | For research use only |
| Application notes | Western blotting: Non-reducing conditions; recommended dilution: 1-2 μg/ml; positive control: MCF-7 cells. Immunoprecipitation: Recommended dilution: 1-4 μg / ml per 100-500 μg of protein in 1 ml lysate. Immunohistochemistry (paraffin sections): Recommended dilution: 1-10 μg/ml; pretreatment: tissue section digestion with pepsin (15 min at RT or 10 min at 37°C, 1 mg / ml Tris-HCl, pH 2.0); positive tissue: breast carcinoma. Immunocytochemistry: Recommended dilution: 1-10 μg/ml. Flow cytometry: Recommended dilution: 1-4 µg/ml. |
| Expiration Date | 12 months from date of receipt. |
Separation of MCF-7 cells (red-filled) from Jurkat cells (black-dashed) in flow cytometry analysis (surface staining) of cellular suspensions of Jurkat and MCF-7 cell lines stained using anti-human CD326 (323/A3) purified antibody (concentration in sample 1 μg/ml, GAM APC).
Western blotting analysis of human CD326 (EpCAM) using mouse monoclonal antibody 323/A3 on lysates of MCF-7 cell line and HEK293T/17 cell line (CD326 non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD326 monoclonal antibody 323/A3 followed by IRDye800-conjugated anti-mouse IgG1 secondary antibody. A specific band was detected for CD326 protein at approximately 40 kDa.
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