CD326 Antibody

SKU: orb43595

Description

Mouse Monoclonal antibody againts EpCAM

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, ICC, IHC-P, IP, WB
• Reactivity: Human
• Application Notes:
  Western blotting: Non-reducing conditions; recommended dilution: 1-2 μg/ml; positive control: MCF-7 cells. Immunoprecipitation: Recommended dilution: 1-4 μg / ml per 100-500 μg of protein in 1 ml lysate. Immunohistochemistry (paraffin sections): Recommended dilution: 1-10 μg/ml; pretreatment: tissue section digestion with pepsin (15 min at RT or 10 min at 37°C, 1 mg / ml Tris-HCl, pH 2.0); positive tissue: breast carcinoma. Immunocytochemistry: Recommended dilution: 1-10 μg/ml. Flow cytometry: Recommended dilution: 1-4 µg/ml.

  Read more...

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 323/A3
• Immunogen: Human breast cancer MCF-7 cells
• Target: CD326
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EPCAM, GA733-2, EGP314, KSA, KS 1/4 antigen, Trop-1, M4S1, DIAR5, MIC18, TROP1

Quick Database Links

Gene Symbol

CD326

UniProt

P16422

NCBI Gene ID

4072