CD303 Antibody

• Catalog Number: orb1742787
• Category: Antibodies
• Description: CD303 is an approximately 38 kDa type II transmembrane glycoprotein with an extracellular C-type lectin domain. It is a specific marker of plasmacytoid dendritic cells, and plays roles in capturing of pathogen-related oligosaccharide-containing antigens by them, and in their presentation to T cells. CD303 also mediates a potent inhibition of interferon alpha/beta production in plasmacytoid dendritic cells, thus it represents a potential target for lupus erythematosus therapy.
• Target: CD303
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified by protein-A affinity chromatography.
• Immunogen: CD303 ectodomain fused with human IgG Fc domain
• UniProt ID: Q8WTT0
• Tested applications: ELISA, FC, ICC, IHC-Fr
• Application notes: Flow cytometry: Recommended dilution: 1-5 μg/ml.
• Clone Number: 15E3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: BDCA2, CLEC4C, DLEC, HECL, CLECSF7, CLECSF11, PRO3
• Note: For research use only
• Entrez: 170482

Flow cytometry multicolor surface staining of human peripheral blood mononuclear cells stained using CD303 (15E3) purified antibody (concentration in sample 3 μg/ml, GAM FITC) and anti-human CD123 (6H6) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD303 positive CD123 positive plasmacytoid dendritic cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD303 (15E3) purified antibody (concentration in sample 3 μg/ml, GAM FITC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD303 (15E3) purified antibody (concentration in sample 3 μg/ml, GAM FITC).