CD303 Antibody (PE)

• Catalog Number: orb1881234
• Category: Antibodies
• Description: CD303 is an approximately 38 kDa type II transmembrane glycoprotein with an extracellular C-type lectin domain. It is a specific marker of plasmacytoid dendritic cells, and plays roles in capturing of pathogen-related oligosaccharide-containing antigens by them, and in their presentation to T cells. CD303 also mediates a potent inhibition of interferon alpha/beta production in plasmacytoid dendritic cells, thus it represents a potential target for lupus erythematosus therapy.
• Target: CD303
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Conjugation: PE
• Reactivity: Human
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Immunogen: CD303 ectodomain fused with human IgG Fc domain
• UniProt ID: Q8WTT0
• Tested applications: FC
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Clone Number: 15E3
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Alternative names: BDCA2, CLEC4C, DLEC, HECL, CLECSF7, CLECSF11, PRO3
• Note: For research use only
• Entrez: 170482
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining pattern of human peripheral blood mononuclear cells using anti-human CD303 (15E3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD123 (6H6) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).

Separation of human CD123 positive CD303 positive plasmacytoid dendritic cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD303 (15E3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD303 (15E3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).