CD28 Antibody

SKU: orb43723

Description

Mouse Monoclonal to CD28.

Research Area

Epigenetics, Stem Cells

Images & Validation

• Tested Applications: FC, ICC, IHC-Fr, IP, WB
• Reactivity: Human, Primate
• Application Notes:
  Flow cytometry: Recommended dilution: 1-12 µg/ml.Western blotting: Recommended dilution: 1-2 µg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone No.: CD28.2
• Immunogen: DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA
• Target: CD28
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TP44

Separation of human CD28 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD28 (CD28.2) purified antibody (concentration in sample 1.6 µg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD28 (CD28.2) purified antibody (concentration in sample 1.6 µg/ml) GAM APC.

Western blotting analysis of human CD28 using mouse monoclonal antibody CD28.2 on lysates of Jurkat cells and THP1 cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD28 was detected around 35-40 kDa.

Quick Database Links

Gene Symbol

CD28

UniProt

P10747

NCBI Gene ID

940