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CD273 Antibody
SKU: orb402782
Description
Research Area
Epigenetics, Immunology
Images & Validation
−Item 1 of 4
| Tested Applications | FC, IHC |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG2a kappa |
| Clone No. | 24F.10C12 |
| Immunogen | human CD273 cDNA |
| Target | CD273 |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−PDCD1LG2, B7DC, Btdc, PDL2, PDCD1L2, bA574F11.2, Butyrophilin, B7-DC,
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Flow cytometry multicolor surface staining pattern of human stimulated (GM-CSF + IL-4) monocytes using anti-human HLA-DR (L243) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD273 (24F.10C12) purified antibody (concentration in sample 0.6 μg/ml) GAM APC.
Flow cytometry multicolor surface staining pattern of human stimulated (GM-CSF + IL-4) monocytes using anti-human CD11c (BU15) Pacific Blue™ antibody (20 μl reagent / 100 μl of peripheral whole blood) and anti-human CD273 (24F.10C12) purified antibody (concentration in sample 0.6 μg/ml) GAM APC.
Separation of human CD11c positive HLA-DR positive cells (red-filled) from CD11c negative HLA-DR negative cells (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) monocytes stained using anti-human CD273 (24F.10C12) purified antibody (concentration in sample 0.6 μg/ml) GAM APC.
Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) monocytes stained using anti-human CD273 (24F.10C12) purified antibody (concentration in sample 0.6 μg/ml) GAM APC.
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Protocol Information
IHC
Immunohistochemistry
FC
Flow Cytometry
CD273 Antibody (orb402782)
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