CD273 Antibody

SKU: orb402782

Description

Mouse monoclonal antibody to CD273

Images & Validation

• Tested Applications: FC, IHC
• Reactivity: Human
• Application Notes:
  Flow cytometry: Recommended dilution: 1-4 µg/ml

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2a kappa
• Clone No.: 24F.10C12
• Immunogen: human CD273 cDNA
• Target: CD273
• Purification: Purified by protein-A affinity chromatography.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

PDCD1LG2, B7DC, Btdc, PDL2, PDCD1L2, bA574F11.2, Butyrophilin, B7-DC,

Flow cytometry multicolor surface staining pattern of human stimulated (GM-CSF + IL-4) monocytes using anti-human HLA-DR (L243) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD273 (24F.10C12) purified antibody (concentration in sample 0.6 μg/ml) GAM APC.

Flow cytometry multicolor surface staining pattern of human stimulated (GM-CSF + IL-4) monocytes using anti-human CD11c (BU15) Pacific Blue™ antibody (20 μl reagent / 100 μl of peripheral whole blood) and anti-human CD273 (24F.10C12) purified antibody (concentration in sample 0.6 μg/ml) GAM APC.

Separation of human CD11c positive HLA-DR positive cells (red-filled) from CD11c negative HLA-DR negative cells (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) monocytes stained using anti-human CD273 (24F.10C12) purified antibody (concentration in sample 0.6 μg/ml) GAM APC.

Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) monocytes stained using anti-human CD273 (24F.10C12) purified antibody (concentration in sample 0.6 μg/ml) GAM APC.

Quick Database Links

Gene Symbol

CD273

UniProt

Q9BQ51

NCBI Gene ID

80380