CD22 Antibody

SKU: orb43979

Description

Mouse Monoclonal to CD22.

Research Area

Cancer, Epigenetics, Stem Cells

Images & Validation

• Tested Applications: FC, IP, WB
• Reactivity: Human, Primate
• Application Notes:
  Flow cytometry: Recommended dilution: 2-4 μg/ml. Western blotting: Recommended dilution: 1-2 μg/ml, non-reducing conditions. The antibody MEM-01 stains only the higher Mw isoform (140 kDa, migrating sometimes as a 125 kDa band) and not the lower Mw isoform (130 kDa, migrating sometimes as a 115 kDa band).

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: MEM-01
• Immunogen: Raji Burkitt's lymphoma cell line
• Target: CD22
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SIGLEC2, SIGLEC-2

Separation of human CD22 positive lymphocytes (red-filled) from human CD22 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD22 (MEM-01) purified antibody (concentration in sample 0.6 μg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD22 (MEM-01) purified antibody (concentration in sample 0.6 μg/ml, GAM APC).

Quick Database Links

Gene Symbol

CD22

UniProt

P20273

NCBI Gene ID

933