Anti-Hu CD1d Purified

• Catalog Number: orb1147809
• Category: Antibodies
• Description: CD1d belongs to CD1 family of transmembrane glycoproteins, associated with beta2 microglobulin, similarly to MHC I molecules. Unlike other CD1 family members, however, CD1d can be also expressed in a non-glycosylated form, which is not associated with beta2 microglobulin. Hence it is not certain how much CD1d plays a role in the presentation of microbial lipid antigens during infection. On the other hand, it is expressed on various antigen presenting cell types. Besides it, CD1d+ thymocytes are involved in the positive selection of sublineage of NKT cells.
• Clonality: Monoclonal
• Clone Number: 51.1
• Tested applications: FC, IHC-Fr, IP, WB
• Reactivity: Human, Primate
• Isotype: Mouse IgG2b kappa
• Immunogen: human CD1d
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD1d
• Entrez: 912
• UniProt ID: P15813
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 4-8 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of human CD1d positive lymphocytes (red-filled) from human CD1d negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD1d (51.1) purified antibody (concentration in sample 5 μg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD1d (51.1) purified antibody (concentration in sample 5 μg/ml, GAM APC).