CD1d Rabbit Polyclonal Antibody

• Catalog Number: orb6186
• Category: Antibodies
• Description: CD1d Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Rat
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse CD1D (51-150/336aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg /Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 36 kDa
• Target: CD1D
• RRID: AB_10921053
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Antigen-presenting glycoprotein CD1d; CD1.1; CD1d;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: mouse spleen cells (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-CD1A antibody (orb6186), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA. Protocol. Primary antibody (orb6186, 1 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-CD1d (orb6186) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36 kD, Observed band size: 61 kD.

Sample: Thymus (Rat) Lysate at 40 ug, Spleen (Mouse) Lysate at 40 ug, Primary: Anti-CD1d (orb6186) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36 kD, Observed band size: 61 kD.