CD163 Antibody

SKU: orb44713

Description

Mouse monoclonal to CD163. CD163, also known as M130, is a member of the scavenger receptor family, accounting for the clearance of hemoglobin-haptoglobin complexes during limited hemolysis, which protects the body, in particular the kidneys, against heme-mediated oxidative damages. It does not have measurable affinity for noncomplexed hemoglobin or haptoglobin. Immunomodulatory role of CD163 has been postulated. CD163 is expressed by cells of the monocyte-macrophage lineage and its extracellular part also circulates in plasma as a soluble protein, especially during sepsis and other conditions affecting macrophage activity, when its level may raise manyfold.

Research Area

Epigenetics, Immunology

Images & Validation

• Tested Applications: FC, IHC-Fr, IP, WB
• Reactivity: Human
• Application Notes:
  Flow cytometry: Recommended dilution: 1-4 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone No.: GHI/61
• Immunogen: Hairy cell leukemia cells
• Target: CD163
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

MM130, SCARI1

Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD163 (GHI/61) purified antibody (concentration in sample 2 µg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD163 (GHI/61) purified antibody (concentration in sample 2 µg/ml) GAM APC.

Quick Database Links

Gene Symbol

CD163

UniProt

Q86VB7

NCBI Gene ID

9332