CD16 Antibody

SKU: orb154424

Description

Mouse monoclonal antibody to CD16 which expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts. It is a receptor for the Fc region of IgG. CD16 is one of only four eukaryotic receptors known to exist natively in both the transmembrane and glycosylphosphatidylinositol isoforms.

Images & Validation

• Tested Applications: FA, FC, IHC-Fr, IP
• Reactivity: Human, Primate
• Application Notes:
  Functional application: In vitro stimulation of NK cell proliferation, blocking of IgG binding and phagocytosis, inhibition of cytotoxic activity, in vivo NK cell depletion. Flow cytometry: Recommended dilution: 6 μg/ml. Immunohistochemistry (frozen sections): Acetone fixation.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone No.: 3G8
• Immunogen: Human neutrophils
• Target: CD16
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FcgammaRIII, IGFR3, FCRIII

Separation of human CD16 positive lymphocytes (red-filled) from CD16 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD16 (3G8) purified antibody (concentration in sample 2 μg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD16 (3G8) purified antibody (concentration in sample 2 μg/ml, GAM APC).

Quick Database Links

Gene Symbol

CD16

NCBI Gene ID

35