Anti-Hu CD15 Purified

• Catalog Number: orb44108
• Category: Antibodies
• Description: Mouse Monoclonal to CD15.
• Clonality: Monoclonal
• Clone Number: MEM-158
• Tested applications: FC, IP
• Reactivity: Human
• Isotype: Mouse IgM
• Immunogen: Human granulocytes
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Purity: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated
• Target: CD15
• RRID: AB_10995533
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Alternative names: Anti-CD15 antibody, anti-Lewis x Blood Group antig
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml.
• Expiration Date: 12 months from date of receipt.

Mass cytometry (surface staining) of human peripheral blood cells (after ammonium chloride red blood cell lysis) with anti-human CD15 (MEM-158) 173Yb. Gated on singlets.

Separation of human neutrophil granulocytes (red-filled) from CD15 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD15 (MEM-158) purified antibody (concentration in sample 0.3 µg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD15 (MEM-158) purified antibody (concentration in sample 0.3 µg/ml) GAM APC.