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Catalog Number | orb545664 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody against CD15 conjugated to PE |
Clonality | Monoclonal |
Clone Number | MMA |
Tested applications | FC |
Reactivity | Human |
Isotype | Mouse IgM kappa |
Immunogen | U937 histiocytic lymphoma cells |
Antibody Type | Primary Antibody |
Purity | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Conjugation | PE |
Target | CD15 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Anti-Lewis x Blood Group antigen antibody, anti-Le Read more... |
Note | For research use only |
Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
Expiration Date | 12 months from date of receipt. |
Flow cytometry analysis (surface staining) of CD15 in human peripheral blood with anti-CD15 (MMA) PE.
Separation of human CD15 positive neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD15 (MMA) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD15 (MMA) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).