Anti-Hu CD15 FITC

• Catalog Number: orb763366
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD15 conjugated to FITC
• Clonality: Monoclonal
• Clone Number: W6D3
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1 kappa
• Immunogen: WERI-RB-1 retinoblastoma cell line
• Antibody Type: Primary Antibody
• Dilution range: FC4 μl reagent / 100 μl of whole blood or 106 cells in a suspension The content of a vial (04 ml) is sufficient for 100 tests
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC
• Target: CD15
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-Lewis x Blood Group antigen antibody, anti-Le
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Separation of human CD15 positive neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD15 (W6D3) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD15 (W6D3) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).