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Catalog Number | orb44110 |
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Category | Antibodies |
Description | Mouse Monoclonal to CD15. |
Clonality | Monoclonal |
Clone Number | MEM-158 |
Tested applications | FC |
Reactivity | Human |
Isotype | Mouse IgM |
Immunogen | Human granulocytes |
Antibody Type | Primary Antibody |
Purity | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Conjugation | FITC |
Target | CD15 |
RRID | AB_11021531 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide |
Alternative names | Anti-CD15 antibody, anti-Lewis x Blood Group antig Read more... |
Note | For research use only |
Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. |
Expiration Date | 12 months from date of receipt. |
Separation of human neutrophil granulocytes (red-filled) from CD15 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD15 (MEM-158) FITC antibody (20 µl reagent / 100 µl of peripheral whole blood).
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD15 (MEM-158) FITC antibody (20 µl reagent / 100 µl of peripheral whole blood).