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Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 4
| Tested Applications | FC, ICC, IHC-Fr, IHC-P, IP, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | Bcl-2/100 |
| Immunogen | Synthetic peptide corresponding to the amino acids 41-54 of human Bcl2 |
| Target | BCL2 |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Bcl-2, PPP1R50
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Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human BCL-2 (Bcl-2/100) purified antibody (concentration in sample 1 μg/ml, GAM APC).
Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human BCL-2 (Bcl-2/100) purified antibody (concentration in sample 1 μg/ml, GAM APC).
Immunohistochemistry staining of human placenta (paraffin-embedded sections) with anti-BCL2 (Bcl-2/100), 10 μg/ml.
Western blotting analysis of human BCL2 using mouse monoclonal antibody Bcl-2/100 on lysates of Jurkat and Raji cells, and 3T3 cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
ICC
Immunocytochemistry
IP
Immunoprecipitation
BCL2 Antibody (orb44688)
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