BCL2 Antibody

SKU: orb44688

Description

Mouse monoclonal antibody to BCL2

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC, ICC, IHC-Fr, IHC-P, IP, WB
• Reactivity: Human
• Application Notes:
  Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.Western blotting: Recommended dilution: 1-2 µg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: Bcl-2/100
• Immunogen: Synthetic peptide corresponding to the amino acids 41-54 of human Bcl2
• Target: BCL2
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Bcl-2, PPP1R50

Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human BCL-2 (Bcl-2/100) purified antibody (concentration in sample 1 μg/ml, GAM APC).

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human BCL-2 (Bcl-2/100) purified antibody (concentration in sample 1 μg/ml, GAM APC).

Immunohistochemistry staining of human placenta (paraffin-embedded sections) with anti-BCL2 (Bcl-2/100), 10 μg/ml.

Western blotting analysis of human BCL2 using mouse monoclonal antibody Bcl-2/100 on lysates of Jurkat and Raji cells, and 3T3 cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody.

Quick Database Links

Gene Symbol

BCL2

UniProt

P10415

NCBI Gene ID

596