Anti-HSP47 Antibody

• Catalog Number: orb1473922
• Category: Antibodies
• Description: Mouse monoclonal antibody to HSP47
• Target: SERPINH1
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Mouse IgG1. Supplied in crude ascites with 0.01% sodium azide.
• Buffer/Preservatives: Mouse IgG1. Supplied in crude ascites with 0.01% sodium azide.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HSP47. The exact sequence is proprietary.
• UniProt ID: P19324, P50454
• Tested applications: IH, WB
• Dilution range: WB (1/1000 - 1/2000), IH (1/50 - 1/200)
• Antibody Type: Primary Antibody
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CBP1; CBP2; HSP47; SERPINH2; Serpin H1; 47 kDa hea
• Note: For research use only
• Entrez: 871, 12406

Western blot analysis of HSP47 expression in rat heart (A) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 46 kD)

Immunohistochemical analysis of HSP47 staining in human skin carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.