HSP47 Antibody (APC)

SKU: orb148126

Description

Mouse monoclonal to Hsp47 (APC). Hsp47 is a chaperone protein, member of the superfamily of serine proteinase inhibitors. Also known as SERPINH1, a serine proteinase inhibitor. It is a stress protein that resides in the endoplasmic reticulum, has an active role on the intracellular process of folding, assembly and secretion of pro-collagens. Recent studies have shown the association of on an increased expression of Hsp47 around fibrotic lesions. The identification of a novel biomarker on cell therapies aimed to reduce the progression of fibrotic diseases, could be used potentially as a universal marker, since Hsp47 binds a single substrate. Type I collagen is fundamental during the healing process after a myocardial infarction. It is critical in the position of collagen-produced cells and the assembly of collagen fibrils..

Research Area

Cell Biology

Images & Validation

• Tested Applications: ICC, IF, WB
• Dilution Range: WB (1:1000), ICC/IF (1:100)
• Reactivity: Human
• Application Notes:
  1 µg/ml was sufficient for detection of HSP47 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 Kappa
• Clone No.: 1C4-1A6
• Immunogen: Human HSP47, full length
• Target: HSP47
• Molecular Weight: 47kDa
• Purification: Protein G Purified
• Conjugation: APC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SERPINH1, HSP47, Heat shock protein 47, Serpin H1, Colligin, Collagen-binding protein, Gp46, Serine protease inhibitor J6, 47 kDa heat shock protein, serine proteinase inhibitor, cysteine proteinase inhibitor, collagen binding protein

Quick Database Links

Gene Symbol

HSP47

UniProt

P50454

NCBI Gene ID

871

RefSeq (Protein):NP_001193943