Anti-HLA-G Purified

• Catalog Number: orb44433
• Category: Antibodies
• Description: Mouse Monoclonal to HLA-G.
• Clonality: Monoclonal
• Clone Number: MEM-G/9
• Tested applications: ELISA, FC, ICC, IHC-Fr, IP
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Recombinant human HLA-G refolded with beta2-microglobulin and peptide.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-5 μg/ml; positive control: JEG-3 human choriocarcinoma cell line. Immunocytochemistry: Recommended dilution: 2-5 μg/ml. For fixation details see: Emadi et al., Biotech Histochem. 2022 Feb;97(2):136-142.ELISA: The antibody MEM-G/9 has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody B2M-01 or with antibody W6/32. Coating antibody 10 μg/ml, detection antibody (biotin or peroxidase conjugate) 1 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: HLA-G
• Entrez: 3135
• UniProt ID: P17693
• RRID: AB_10993141
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-HLA-G antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml; positive control: JEG-3 human choriocarcinoma cell line. Immunocytochemistry: Recommended dilution: 2-5 μg/ml. ELISA: The antibody MEM-G/9 has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody B2M-01 or with antibody W6/32. Coating antibody 10 μg/ml, detection antibody (biotin or peroxidase conjugate) 1 μg/ml.
• Expiration Date: 12 months from date of receipt.

Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9) and protein G. HLA-G was detected by anti-human HLA-G (clone 4H84) and goat anti-mouse HRP in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).

Separation of HLA-G trasnfected LCL cells (red-filled) from K562 cells (black-dashed) in flow cytometry analysis (surface staining) stained using anti-human HLA-G (MEM-G/9) purified antibody (concentration in sample 0.3 µg/ml, GAM APC).