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HLA-G Antibody
SKU: orb1262443
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Description
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−Item 1 of 6
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Immunogen | This HLA-G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-89 amino acids from the Central region of human HLA-G. |
| Target | HLA-G |
| Molecular Weight | 38 kDa |
| Purification | This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−HLA class I histocompatibility antigen, alpha chain G, HLA G antigen, MHC class I antigen G, HLA-G, HLA-60, HLAG
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Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Raji whole cell lysate Lane 3: Ramos whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of lysate from human placenta tissue lysate, using HLA-G Antibody) at 1:1000 at each lane.
Western blot analysis of HLA-G Antibody in NCI-H460 cell line lysates (35 ug/lane)
Western blot analysis of HLA-G using rabbit polyclonal HLA-G Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-G gene.
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HLA-G Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
HLA-G Antibody (orb1262443)
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