Anti-HLA-G Purified

• Catalog Number: orb44432
• Category: Antibodies
• Description: Mouse Monoclonal to HLA-G.
• Target: HLA-G
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purity: Purified by protein-A affinity chromatography.
• Purification: Purified by protein-A affinity chromatography.
• Immunogen: Recombinant human HLA-G denaturated heavy chain.
• UniProt ID: P17693
• RRID: AB_10994306
• Tested applications: WB
• Application notes: Western blotting: Recommended dilution: 1-2 μg/ml.
• Antibody Type: Primary Antibody
• Clone Number: MEM-G/4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-HLA-G antibody
• Note: For research use only
• Entrez: 3135

Western blotting analysis of human HLA-G using mouse monoclonal antibody MEM-G/4 on lysates of JEG-3 cell line and LNCaP cell line (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody anti-HLA-G followed by IRDye800-conjugated anti-mouse secondary antibody. Specific bands were detected for HLA-G at approximately 42-46 kDa.