Anti-HLA-G Purified

• Catalog Number: orb44431
• Category: Antibodies
• Description: Mouse Monoclonal to HLA-G.
• Clonality: Monoclonal
• Clone Number: MEM-G/2
• Tested applications: IHC-Fr, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Bacterially expressed recombinant HLA-G heavy chain (denatured).
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: HLA-G
• Entrez: 3135
• UniProt ID: P17693
• RRID: AB_10999408
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-HLA-G antibody
• Note: For research use only
• Application notes: Western blotting: Recommended dilution 1-2 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blotting analysis of HLA-G in LCL-HLA-G transfectants using anti-HLA-G (MEM-G/2).

Immunohistochemistry staining of kidney allograft biopsy (paraffin-embedded sections) with anti-human HLA-G (MEM-G/2).

Western blotting analysis of human HLA-G using mouse monoclonal antibody MEM-G/2 on lysates of JEG-3 cell line and LNCaP cell line (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody anti-HLA-G followed by IRDye800-conjugated anti-mouse secondary antibody. HLA-G was detected at approximately 40 kDa.