HLA-G Antibody

SKU: orb43617

Description

Mouse Monoclonal to HLA-G.

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, FC, ICC, IHC-Fr, IHC-P, WB
• Reactivity: Human
• Application Notes:
  Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining. Immunohistochemistry (frozen sections): Recommended dilution: 10 μg/ml; positive tissue: placenta.Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: placenta. ELISA: Positive control: HeLa/HLA-G5 transfectants cell lysate, HeLa/HLA-G5 cell supernatant; negative control: HeLa cell lysate. The antibody 5A6G7 has been tested as the capture antibody in a sandwich ELISA for analysis of soluble HLA-G in combination with antibody W6/32 (cat. no. orb44577). Western blotting: Positive control: JEG-3 cell lysate, reducing conditions, 12% AA SDS-PAGE.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 5A6G7
• Immunogen: C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin.
• Target: HLA-G
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blotting analysis of whole cell lysate of HLA-G stable transfectants (various splice variants) using anti-human HLA-G (5A6G7). Lane 1: M8 cell line transfected with empty vector; Lane 2: M8 cell line transfected with HLA-G1; Lane 3: M8 cell line transfected with HLA-G5; Lane 4: M8 cell line transfected with HLA-G6.

Quick Database Links

Gene Symbol

HLA-G

UniProt

P17693

NCBI Gene ID

3135