Anti-HLA-G Purified

• Catalog Number: orb43590
• Category: Antibodies
• Description: Mouse Monoclonal to HLA-G.
• Clonality: Monoclonal
• Clone Number: 2A12
• Tested applications: ELISA, FC, IHC-Fr, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Purity: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated
• Target: HLA-G
• Entrez: 3135
• UniProt ID: P17693
• RRID: AB_10990078
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-HLA-G antibody
• Note: For research use only
• Application notes: Flow cytometry: Excellent for intracellular staining; recommended dilution: 1-4 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of HLA-G transfected HEK-293 cells (red-filled) from HLA-G negative debris (black-dashed) in flow cytometry analysis (intracellular staining) of HLA-G transfected HEK-293 cells using anti-human HLA-G (2A12) purified antibody (concentration in sample 4 µg/ml) GAM APC.

Flow cytometry intracellular staining pattern of HLA-G transfected HEK-293 cells using anti-human HLA-G (2A12) purified antibody (concentration in sample 4 µg/ml) GAM APC.