Anti-HLA-DR/DP Purified

• Catalog Number: orb44064
• Category: Antibodies
• Description: Mouse Monoclonal to HLA-DR.
• Clonality: Monoclonal
• Clone Number: MEM-136
• Tested applications: FC, IHC-P, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: PHA-activated peripheral blood lymphocytes.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-4 μg/ml. Western blotting: Non-reducing conditions.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: HLA-DR/DP
• RRID: AB_10993764
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-HLA-DR antibody, anti-DP antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml. Western blotting: Recommended dilution: 1 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blotting analysis of MHC Class II in whole cell lysate of RAJI human Burkitt lymphoma cell line using anti-human HLA-DR+DP (MEM-136). The antibody MEM-136 recognizes β-chain of HLA-DR antigen under non-reducing conditions. Lane 1: immunostaining with anti-human HLA-DR+DP (MEM-136); Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06; orb43531)

Separation of human HLA-DR/DP positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human HLA-DR/DP (MEM-136) purified antibody (concentration in sample 4 µg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human HLA-DR/DP (MEM-136) purified antibody (concentration in sample 4 µg/ml) GAM APC.

Immunohistochemistry staining of human small intestine (paraffin-embedded sections) with anti-HLA-DR+DP (MEM-136).

Anti-HLA-DR/DP (clone MEM-136) works in WB application under reducing and non-reducing conditions. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Raji and Jurkat cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 10% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody MEM-136 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed. HLA-DR/DP molecules were detected at ~25 kDa in Raji cell line.