Anti-HLA-DR/DP Purified

• Catalog Number: orb43864
• Category: Antibodies
• Description: Mouse Monoclonal to HLA-DR.
• Clonality: Monoclonal
• Clone Number: HL-38
• Tested applications: FC, IHC-P, IP, WB
• Reactivity: Human, Porcine
• Isotype: Mouse IgG2a
• Immunogen: Raji Burkitt's lymphoma cell line
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Dilution range: Western blotting: Non-reducing conditions. Flow cytometry: Recommended dilution: 1-4 µg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: HLA-DR/DP
• RRID: AB_10992473
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-HLA-DR antibody, anti-DP antibody
• Note: For research use only
• Application notes: Western blotting: Recommended dilution: 1 µg/ml Flow cytometry: Recommended dilution: 1-4 µg/ml
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis (surface staining) of human peripheral blood with anti-HLA-DR+DP (HL-38) PE.

Immunohistochemistry staining of human small intestine (paraffin-embedded sections) with anti-HLA-DR+DP (HL-38).

Anti-HLA-DR/DP (clone HL-38) works in WB application under reducing and non-reducing conditions. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Raji and Jurkat cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 10% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with mouse IgG2a monoclonal antibody HL-38 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed. HLA-DR/DP molecules were detected at ~25 kDa in Raji cell line.