Anti-HLA-B7 Purified

• Catalog Number: orb154379
• Category: Antibodies
• Description: Mouse monoclonal antibody to HLA-B7 which is allele of human HLA class I major histocompatibility (MHC) antigen that indicates higher risk of breast cancer and cervical cancer. The HLA-B7 antigen (11 alleles) is expressed in 22% of healthy Caucasian individuals. In HLA-B7 , B7 is a risk factor for cervical cancer, sarcoidosis, and early-onset spondylarthropathies.
• Clonality: Monoclonal
• Clone Number: BB7.1
• Tested applications: FC
• Reactivity: Human, Primate
• Isotype: Mouse IgG1
• Immunogen: Papain solubilised HLA-A2, B7
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: HLA-B7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of human lymphocytes of HLA-B7 positive blood donor (red-filled) from human lymphocytes of HLA-B7 negative blood donor (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood samples stained using anti-HLA-B7 (BB7.1) purified antibody (concentration in sample 2 μg/ml, GAM APC).

Flow cytometry surface staining patterns of human peripheral whole blood of HLA-B7 positive (left) and negative (right) blood donors stained using anti-HLA-B7 (BB7.1) purified antibody (concentration in sample 2 μg/ml, GAM APC).