HLA-B7 Antibody (PE)

SKU: orb402821

Description

Mouse monoclonal antibody against HLA-B7 conjugated to PE

Images & Validation

• Tested Applications: FC
• Reactivity: Human, Primate
• Application Notes:
  Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: BB7.1
• Immunogen: Papain solubilised HLA-A2, B7
• Target: HLA-B7
• Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Separation of human lymphocytes of HLA-B7 positive blood donor (red-filled) from human lymphocytes of HLA-B7 negative blood donor (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood samples stained using anti-HLA-B7 (BB7.1) PE antibody (10 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry surface staining patterns of human peripheral whole blood of HLA-B7 positive (left) and negative (right) blood donors stained using anti-HLA-B7 (BB7.1) PE antibody (10 µl reagent / 100 µl of peripheral whole blood).

Quick Database Links

Gene Symbol

HLA-B7

NCBI Gene ID

35