Anti-HLA-A2 [BB7.2]

• Catalog Number: orb1906406
• Category: Antibodies
• Description: Anti-HLA-A2 [BB7.2]
• Target: HLA-A2
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2b
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Immunogen: The original antibody was generated by immunizing BALB/c mice with papain solubilized HLA-A2 and B7 antigens.
• UniProt ID: P04439
• Tested applications: ELISA, FC, IF, IHC, WB
• Clone Number: BB7.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HLA-A; Human leukocyte antigen A; HLA class I hist
• Note: For research use only

Immunofluorescence staining of Daudi cells with anti-HLA-A2 antibody BB7.2. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of BB7.2 (orb1906407) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb1906407, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Immunofluorescence staining of Daudi cells with anti-HLA-A2 antibody BB7.2. Immunofluorescence analysis of paraformaldehyde-fixed Daudi cells on Shi-fix™ coverslips stained with the mouse IgG2b version of BB7.2 (orb1906406) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb1906406, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256459) followed by staining with Alexa Fluor® 488 secondary antibody.

Western blot using anti-HLA-A2 antibody BB7.2. Daudi cells lysate (35 µg protein in RIPA buffer) was resolved via SDS-PAGE, and the subsequent blot was probed with the chimeric rabbit version of BB7.2 (orb1906407) at 0.3 µg/ml before detection using an appropriate secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.