Anti-HLA-A2 [BB7.2]

SKU: orb1906406

Description

Anti-HLA-A2 [BB7.2]

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: BB7.2
• Immunogen: The original antibody was generated by immunizing BALB/c mice with papain solubilized HLA-A2 and B7 antigens.
• Target: HLA-A2
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HLA-A; Human leukocyte antigen A; HLA class I histocompatibility antigen; A-2 alpha chain; MHC class I antigen A-2 HLA-A; HLA class I histocompatibility antigen A-28 alpha chain; HLA class I histocompatibility antigen; A-28 alpha chain; MHC class I antigen A-28

Immunofluorescence staining of Daudi cells with anti-HLA-A2 antibody BB7.2. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of BB7.2 (orb1906407) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb1906407, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Immunofluorescence staining of Daudi cells with anti-HLA-A2 antibody BB7.2. Immunofluorescence analysis of paraformaldehyde-fixed Daudi cells on Shi-fix™ coverslips stained with the mouse IgG2b version of BB7.2 (orb1906406) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb1906406, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256459) followed by staining with Alexa Fluor® 488 secondary antibody.

Western blot using anti-HLA-A2 antibody BB7.2. Daudi cells lysate (35 µg protein in RIPA buffer) was resolved via SDS-PAGE, and the subsequent blot was probed with the chimeric rabbit version of BB7.2 (orb1906407) at 0.3 µg/ml before detection using an appropriate secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.

Quick Database Links

Gene Symbol

HLA-A2

UniProt

P04439