Histone H3 Antibody

• Catalog Number: orb339084
• Category: Antibodies
• Description: The Histone H3 Antibody is suitable for WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone H3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Bovine, Gallus, Human, Mouse, Porcine, Rat, Zebrafish
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone H3. The exact sequence is proprietary.
• UniProt ID: P84243, Q71DI3, Q6NXT2, P68431
• Tested applications: WB
• Dilution range: WB: 1:500-1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HI
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 8353, 8356, 8357, 3020, 440093, 3021, 8355, 126961, 8351, 8352, 8354, 333932, 8968, 8350, 653604, 8358

Western blot analysis of Histone H3 expression in NIH3T3 (A) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 17 kD)

Direct ELISA antibody dose-response curve using Anti-Histone H3 (DiMethyl-K9) Antibody. Antigen (methyl-peptide and non-methyl-peptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.