GST tag Antibody

SKU: orb44500

Description

Mouse Monoclonal to GST.

Images & Validation

• Tested Applications: IP, WB
• Reactivity: All
• Application Notes:
  Western blotting: Recommended dilution: 1 μg/ml; positive control: GST fusion protein, reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone No.: S-tag-05
• Immunogen: GST fusion protein
• Target: GST tag
• Purification: Purified by protein-A affinity chromatography.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Western blotting analysis of isolation of Glutathione-S-Transferase (GST) overexpressed in E. coli. Immunostained by anti-GST (S-tag-05). Lane 1: affinity purification of GST from bacterial cell lysate using commercial sorbent Glutathione-Sepharose; Lane 2: affinity purification of GST from bacterial cell lysate using immunosorbent prepared from anti-GST (S-tag-02).

Quick Database Links

Gene Symbol

GST tag

NCBI Gene ID

35