GST tag Rabbit Polyclonal Antibody

• Catalog Number: orb13452
• Category: Antibodies
• Description: GST tag Rabbit Polyclonal Antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: All
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: Recombinant full length GST tag protein
• RRID: AB_10767966
• Tested applications: ELISA, WB
• Dilution range: WB=1:20000-60000, ELISA=1:1000-5000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: GST PI protein; Glutathione S Transferase; Recombi
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: GST-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: orb1516792) at 4 ug, Primary: Anti-GST Tag (orb13452) at 1/1000 ~ 1/10000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 41 kD.

Transformed (GST-tag or EV) E. coli cells lysates were subjected to SDS-PAGE followed by WB with orb13452 (Anti-GST) at dilution of 1:60000 incubated at 4°C overnight.