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Anti-GRP94 Antibody

Catalog Number: orb214702

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb214702
CategoryAntibodies
DescriptionRabbit polyclonal antibody to HSP90B1
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GRP94. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetHSP90B1
Entrez7184
UniProt IDP14625
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti GRP94 antibody, anti TRA1 antibody, anti Endo
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-GRP94 Antibody

Western blot analysis of GRP94 expression in K562 (A), A549 (B), SP20 (C), PMVEC (D) whole cell lysates. (Predicted band size: 92 kD; Observed band size: 100 kD)

Anti-GRP94 Antibody

Immunohistochemical analysis of GRP94 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-GRP94 Antibody

Immunofluorescent analysis of GRP94 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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    ELISA,  IF,  IHC-P,  WB

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    ELISA,  IF,  IHC,  WB

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