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Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, FC, IF, IHC, IP, WB |
|---|---|
| Dilution Range | WB: 1:1000-1:4000; IP: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | heat shock protein 90kDa beta(Grp94), member 1 |
| Target | HSP90B1 |
| Molecular Weight | 94 kDa |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | Immunogen affinity purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ECGP, Endoplasmin, GP96, gp96 homolog, GRP 94, GRP94, HSP90B1, TRA1, Tumor rejection antigen 1
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A549 cells were subjected to SDS PAGE followed by western blot with orb627523 (HSP90B1 antibody) at dilution of 1:1000.
Immunofluorescent analysis of HepG2 cells, using HSP90B1 antibody orb627523 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-GRP94 (IP: orb627523, 3 ug; Detection: orb627523 1:1000) with mouse liver tissue lysate 4000 ug.
Immunohistochemistry of paraffin-embedded human breast cancer using orb627523 (HSP90B1 antibody) at dilution of 1:50.
Quick Database Links
Gene Symbol
HSP90B1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
HSP90B1 Rabbit Polyclonal Antibody (orb627523)
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