Anti-GPR49 Antibody

• Catalog Number: orb1474144
• Category: Antibodies
• Description: Mouse monoclonal antibody to GPR49
• Target: LGR5
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: This antibody is purified through a protein G column.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPR49. The exact sequence is proprietary.
• UniProt ID: O75473
• Tested applications: FC, WB
• Dilution range: WB (1/500 - 1/1000), FC (1/10 - 1/50)
• Antibody Type: Primary Antibody
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: GPR49; GPR67; Leucine-rich repeat-containing G-pro
• Note: For research use only
• Entrez: 8549

Western blot analysis of GPR49 expression in K562 (A) whole cell lysates. (Predicted band size: 99 kD; Observed band size: 110 kD)

Flow cytometric analysis of Hela cells using Anti-GPR49 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.