Anti-GNG12 Antibody

• Catalog Number: orb393040
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GNG12.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Primate, Rabbit, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GNG12. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500:1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: GNG12
• Entrez: 14701, 55970
• UniProt ID: Q9UBI6, Q9DAS9
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Guanine nucleotide-binding protein G(I)/G(S)/G(O)
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GNG12 expression in HepG2 (A), mouse lung (B), rat lung (C) whole cell lysates. (Predicted band size: 8 kD; Observed band size: 8 kD)

Immunofluorescent analysis of GNG12 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).