GNG12 Antibody (C-Term)

• Catalog Number: orb1925950
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This GNG12 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 37-68 amino acids from human GNG12.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q9UBI6
• MW: 8006 Da
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:2000, IHC-P: 1:25
• Antibody Type: Primary Antibody
• Clone Number: RB54056
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Guanine nucleotide-binding protein G(I)/G(S)/G(O)
• Note: For research use only

All lanes: Anti-GNG12 Antibody (C-Term) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: DU145 whole cell lysate. Lane 3: mouse brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 8 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining GNG12 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.