Anti-GLS2 Antibody

• Catalog Number: orb1473639
• Category: Antibodies
• Description: Mouse monoclonal antibody to GLS2
• Target: GLS2
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: This antibody is purified through a protein G column.
• Immunogen: Recombinant fusion protein of human GLS2. The exact sequence is proprietary.
• UniProt ID: Q9UI32, Q571F8
• Tested applications: IH, WB
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/200)
• Antibody Type: Primary Antibody
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: GA; Glutaminase liver isoform, mitochondrial; GLS;
• Note: For research use only
• Entrez: 27165, 216456

Western blot analysis of GLS2 expression in human brain (A), mouse liver (B) whole cell lysates. (Predicted band size: 66 kD; Observed band size: 66 kD)

Immunohistochemical analysis of GLS2 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.