GFRA1 Antibody

• Catalog Number: orb378073
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GFRA1.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant fusion protein of human GFRA1
• UniProt ID: P97785, P56159, Q62997
• Tested applications: IF, IHC, WB
• Dilution range: WB: 1:500-2000, IF/ICC: 1:50-200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: GDNFRA; RETL1; TRNR1; GDNF family receptor alpha-1
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 14585, 2674, 25454
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GFRA1 expression in MCF7 (A), A549 (B), SW480 (C) whole cell lysates. (Predicted band size: 50; 51 kD; Observed band size: 55 kD)

Immunohistochemical analysis of GFRA1 staining in human thyroid cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of GFRA1 staining in SW480 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.