Anti-GFAP Antibody

• Catalog Number: orb1473999
• Category: Antibodies
• Description: Mouse monoclonal antibody to GFAP
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IF, IH, WB
• Reactivity: Human
• Immunogen: Recombinant fusion protein of human GFAP. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB (1/500 - 1/2000), IH (1/100 - 1/400), IF/IC (1/10 - 1/50)
• Form/Appearance: Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: GFAP
• Entrez: 2670
• UniProt ID: P14136
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Glial fibrillary acidic protein; GFAP
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GFAP expression in human brain (A), human cerebellum (B) whole cell lysates. (Predicted band size: 49 kD; Observed band size: 45-50 kD)

Immunohistochemical analysis of GFAP staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of GFAP staining in brain tissue cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).