You have no items in your shopping cart.
Cart summary
Item 1 of 3
Item 1 of 3
Anti-GCAT Antibody
Catalog Number: orb666752
| Catalog Number | orb666752 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to GCAT |
| Target | GCAT |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Immunogen | Recombinant fusion protein of human GCAT. The exact sequence is proprietary. |
| UniProt ID | O75600, O88986 |
| Tested applications | IF, IH, WB |
| Dilution range | WB: 1-500-2000 |
| Antibody Type | Primary Antibody |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-KBL antibody, anti-2-amino-3-ketobutyrate coe Read more... |
| Note | For research use only |
| Entrez | 26912, 23464 |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of GCAT expression in mouse eye (A) whole cell lysates. (Predicted band size: 45; 47 kD; Observed band size: 45 kD)
Immunohistochemical analysis of GCAT staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of GCAT staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
