GCAT Antibody (Center)

• Catalog Number: orb1929207
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This GCAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155-181 amino acids from the Central region of human GCAT.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: O75600
• MW: 45285 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB18219
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: 2-amino-3-ketobutyrate coenzyme A ligase, mitochon
• Note: For research use only
• NCBI: NP_055106.1, NP_001165161.1
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GCAT antibody (Center) in mouse liver tissue lysates (35 ug/lane). GCAT (arrow) was detected using the purified Pab.

Western blot analysis of GCAT (arrow) using rabbit polyclonal GCAT Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GCAT gene.

Flow cytometric analysis of HepG2 cells using GCAT Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human brain reacted with GCAT Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.