GAPDH Rabbit Monoclonal Antibody

SKU: orb547170

Description

Anti-GAPDH Rabbit Monoclonal Antibody. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Images & Validation

• Tested Applications: FC, ICC, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB 1:5000-1:50000IHC 1:100-1:500ICC/IF 1:100-1:250IP 1:50FC 1:50. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: BH-7
• Immunogen: A synthesized peptide derived from human GAPDH
• Molecular Weight: 58 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Concentration: 0.5mg/ml
• Disclaimer: For research use only

Alternative Names

Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; 1.2.1.12; Peptidyl-cysteine S-nitrosylase GAPDH; 2.6.99.-; GAPDH; GAPD; CDABP0047, OK/SW-cl.12

WB analysis of GAPDH expression in (1) HeLa Cell; (2)Jurkat Cell; (3)Mouse kidney lysate; (4) Mouse spleen lysate; (5) RAW 264.7 Cell; (6) Rat brain lysate with GAPDH Antibody.

Immunofluorescent analysis of HeLa cells, using GAPDH Antibody.

Immunohistochemical analysis of paraffin-embedded (1) Rat colon; (2) Mouse testis; (3) Human uterus; (4) Human kidney, using GAPDH Antibody.

Quick Database Links

UniProt

P04406