Anti-GAP43 Antibody

• Catalog Number: orb1474638
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GAP43
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein corresponding to human GAP43. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: GAP43
• Entrez: 2596, 29423, 14432
• UniProt ID: P06837, P07936, P17677
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Neuromodulin; Axonal membrane protein GAP-43; Grow
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GAP43 expression in U251MG (A), mouse heart (B), rat brain (C) whole cell lysates. (Predicted band size: 24; 28 kD; Observed band size: 43 kD)

Immunofluorescent analysis of GAP43 staining in SHSY5Y cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).