GAP43 Antibody

• Catalog Number: orb256550
• Category: Antibodies
• Description: The GAP43 Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human GAP43. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Bovine, Gallus, Human, Monkey, Mouse, Rat, Zebrafish
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human GAP43. The exact sequence is proprietary.
• UniProt ID: P06837, P17677, P07936
• Tested applications: IF, IHC, WB
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Neuromodulin; Axonal membrane protein GAP-43; Grow
• Research Area: Developmental Biology, Epigenetics
• Note: For research use only
• Entrez: 14432, 29423, 2596

References

Masoumeh Pourhadi,Hakimeh Zali,Rasoul Ghasemi,Mehrdad Faizi,Faraz Mojab,Mina Soufi Zomorrod Restoring Synaptic Function: How Intranasal Delivery of 3D-Cultured hUSSC Exosomes Improve Learning and Memory Deficits in Alzheimer's Disease Mol Neurobiol, (2023)

PubMed: 38010560

Applications

IF

Reactivity

Rat

Western blot analysis of GAP43 expression in HEK293T (A), Hela (B), A549 (C) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 48 kD)

Immunohistochemical analysis of GAP43 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of GAP43 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).