Anti-FOXP2 [RAB-S249]

• Catalog Number: orb1089942
• Category: Antibodies
• Description: Rabbit polyclonal antibody to FOXP2
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: RAB-S249
• Tested applications: ELISA, FC, IP
• Reactivity: Human
• Isotype: Human IgG
• Immunogen: This antibody was obtained by recombinant antibody (rAb) phage display recognizing FOXP2 protein under non-denaturing conditions.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: FOXP2
• UniProt ID: O15409
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CAG repeat protein 44; Forkhead box protein P2; Tr
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the anti-FOXP2 antibody RAB-S249. HepG2 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG1 version of RAB-S249 (orb1089942, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed HeLa cells with anti-FOXP2 antibody RAB-S249. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of RAB-S249 (orb1089942) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom orb1089942, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.