Anti-FOXP2 [RAB-S249]

SKU: orb1089919

Description

Human polyclonal antibody to FOXP2

Images & Validation

• Tested Applications: ELISA, FC, IP
• Reactivity: Human

Key Properties

• Host: Human
• Clonality: Monoclonal
• Isotype: Fab fragment
• Clone No.: RAB-S249
• Immunogen: This antibody was obtained by recombinant antibody (rAb) phage display recognizing FOXP2 protein under non-denaturing conditions.
• Target: FOXP2
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

CAG repeat protein 44; Forkhead box protein P2; Trinucleotide repeat-containing gene 10 protein; anti-FOXP2-RAB-S249; S0014_B01_HR7934B.006_A10

Flow cytometry using the anti-FOXP2 antibody RAB-S249. HepG2 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG1 version of RAB-S249 (orb1089942, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed HeLa cells with anti-FOXP2 antibody RAB-S249. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of RAB-S249 (orb1089942) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom orb1089942, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

FOXP2

UniProt

O15409