fibrillarin/FBL (aa99-110) Antibody

• Catalog Number: orb12327
• Category: Antibodies
• Description: Goat polyclonal to FBL.
• Target: fibrillarin / FBL (aa99-110)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: CRGKEDALVTKN
• MW: 33.8
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml
• Application notes: WB: Approx 35kDa band observed in lysates of cell line HEK293 (calculated MW of 33.8kDa according to NP_001427.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti FBL antibody
• Note: For research use only
• Entrez: 2091

Western blot analysis of HEK293 lysate using FBL antibody