Anti-FGF23 Antibody

• Catalog Number: orb1878954
• Category: Antibodies
• Description: Rabbit polyclonal antibody to FGF23
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein of human FGF23. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Entrez: 64654, 170583, 8074
• UniProt ID: Q8VI82, Q9EPC2, Q9GZV9
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Alternative names: HYPF; Fibroblast growth factor 23; FGF-23; Phospha
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of FGF23 expression in mouse brain (A), rat brain (B) whole cell lysates. (Predicted band size: 27 kD; Observed band size: 28 kD)

Immunofluorescent analysis of FGF23 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).