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| Catalog Number | orb770495 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to FGF-23 |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human FGF23. AA range:151-200 |
| Concentration | 1 mg/ml |
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications. |
| Conjugation | Unconjugated |
| MW | 27 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-FGF23 antibody, anti-HYPF antibody, anti-Fibr Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of A549. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.
Immunofluorescence analysis of HUVEC cells, using FGF23 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night.
